課題番号 ：S-16-KU-0060
利用形態 ：機器利用
利用課題名（日本語） ：薬物担体としての高親和性ヒト血清アルブミン結合ペプチドの設計
Program Title (English) ：Designing a high-affinity human serum albumin binding peptide as drug carrier.
利用者名（日本語） ：エルナズ　ナカーエイ, 森　健
Username (English) ：E. Nakhaei, T. Mori
所属名（日本語） ：九州大学大学院システム生命科学府
Affiliation (English) ：Systems Life Science, Kyushu University

１．概要（Summary ）
Binding of a synthesized peptide to serum albumin (Fig1.) was intended to be measured.

Fig 1. Synthesized hormone peptide carrier for shwoing high binding affinity to HSA hydrophobic pockets.

２．実験（Experimental）
MicroCal VP-ITC, 等温滴定型カロリメトリー（ITC）
Titration of fatty acid into HSA solution.

３．結果と考察（Results and Discussion）
At first titration of 0.01 mM HSA with 0.1 mM of Myristic acid was run. The produced differential power (DP) between the reference and sample cell was interpreted to be very low (Fig.2), so for the next run concentrations were increased. As of titration of 0.05 mM HSA with 1 mM Myristic acid again the produced DP could not be explained (Fig 3.). For clarification of the generated heat by addition of only methanol (Myristic acid solvent) into the HSA solution, titration of 0.05 mM HSA solution with methanol alone was conducted. Result showed almost zero DP (Fig.4). To sum up, so far we could not get meaningful data, so we have to optimize our protocol of using ITC for studying interaction of the small hydrophobic molecules with HSA.
Fig. 2

Fig. 3

Fig. 4

４．その他・特記事項（Others）
なし。

５．論文・学会発表（Publication/Presentation）
なし。

６．関連特許（Patent）
なし。