課題番号 ：S-16-NM-0017
利用形態 ：機器利用
利用課題名（日本語） ：ナノ材料の合成と生物医学的応用
Program Title (English) ：Synthesis and biomedical application of nanomaterials
利用者名（日本語） ：王秀鵬1)
Username (English) ：Xiupeng Wang1)
所属名（日本語） ：1)産業技術総合研究所 (AIST)
Affiliation (English) ：1) National Institute of Advanced Industrial Science and Technology

１．概要（Summary ）

We used NIMS Molecule & Material Synthesis Platform to study the interaction between different nanomaterials and cells. The particle size and surface charge, drug/protein adsorption/release, cellular uptake of the different nanomaterials were studied.

２．実験（Experimental）

Equipments used in this year include レーザーゼータ電位計 ELSZ-1000, セルアナライザー SP6800, 共焦点レーザー顕微鏡 TCS SP5, etc. The experiment operation was guided by the specialist in NIMS Molecule & Material Synthesis Platform. Operation of the equipment was in accordance with the guidebook of the equipment.

３．結果と考察（Results and Discussion）

We synthesized fluorine-substituted hydroxyapatite nanoparticles (FHA) in National Institute of Advanced Industrial Science and Technology (AIST). Part of evaluation of these FHA nanoparticles was supported by NIMS Molecule & Material Synthesis Platform in 2016.

For example, using共焦点レーザー顕微鏡 TCS SP5, we studied effect of FHA on cellular uptake of a fluorescein conjugates protein (fluorescein conjugates of ovalbumin). From the results, free protein was slightly engulfed by bone marrow dentritic cells in vitro. In contrast, FHA nanoparticles loaded with protein were efficiently engulfed by bone marrow dentritic cells in vitro.

４．その他・特記事項（Others）
なし

５．論文・学会発表（Publication/Presentation）
1) Xiupeng Wang, Xia Li, Atsuo Ito, Yohei Watanabe, Noriko M. Tsuji. Rod-shaped and fluorine-substituted hydroxyapatite free of molecular immunopotentiators stimulates anti-cancer immunity in vivo. Chem. Commun., 2016, 52, 7078-7081.

６．関連特許（Patent）
なし