課題番号 ：　　　S-15-NM-0047
利用形態 ：共同研究
利用課題名（日本語） ：
ProgramTitle(English) ：Development of novel nano-scaled delivery system for CpG-ODN
利用者名（日本語） ：
Username(English) ：Song Chen
所属名（日本語）：
Affiliation (English) ：Taiyuan University of Technology

１．概要（Summary ）
CpG-ODN is one type of typical DNA immunoadjuvants. However, its negative nature results in a poor cellular uptake and a low immunostimulatory response. It has been demonstrated that the positively charged nano-scaled delivery system well complexed with CpG-ODN and effectively delivered CpG-ODN into immune cells with enhanced cellular uptake and immunostimulatory response. Silica nanotubes are well biocompatible and provide the void space for delivery of drug and biological growth.
In this study, the positively charged rhodamine-labeled silica nanotubes (RhSiNT) were synthesized and then employed as novel delivery system for CpG-ODN.

２．実験（Experimental）
Silica nanotubes were prepared via a collagen-templated sol-gel route with a subsequent removal of collagen template in an acidic condition. They were then soaked in a sol-gel precursor system consisting of ethanol, tetraethoxysilane, aminopropyltriethoxysilane, and rhodamine B isothiocyanate to produce rhodamine-labeled silica nanotubes (RhSiNT). RhSiNT was then complexed with CpG-ODN2006x3 to produce RhSiNT/CpG-ODN complex. In vitro immunostimulatory response was evaluated through incubation of RhSiNT/CpG-ODN complex with RAW 264.7 macrophage cells.

３．結果と考察（Results and Discussion）
Size and morphology of RhSiNT were observed under a scanning electron microscopy. RhSiNT was tubular in microstructure and had the diameter of 300-400 nm. RhSiNT was positively charged with a zeta potential of +39.6 mV and condensed CpG-ODN via an electrostatic interaction to produce RhSiNT/CpG-ODN complex (0.06 µg CpG-ODN/µg RhSiNT). After incubation with Raw cells for 16 h, the amount of IL-6 for RhSiNT/CpG-ODN complex was a slight higher than that for naked CpG-ODN, indicating that the present silica nanotubes enhanced immunostimulatory response of CpG-ODN.

４．その他・特記事項（Others）
なし

５．論文・学会発表（Publication/Presentation）
なし

６．関連特許（Patent）
なし