Subject Number : S-17-NM-0040
Support Type : 機器利用
Proposal Title (English) : Altering in cell cycle of A431 human epithelial cells by polystyrene nanoparticles
Username (English) : Le Thi Minh Phuc
Affiliation (English) : Graduate School of Advanced Science and Engineering, Waseda University

1. Summary
Polystyrene nanoparticles can be internalized into A431 cells. This process could be effected by some factors such as dose, size, cell type… Therefore this research will be focused on the effect of EGF on cellular uptake of polystyrene nanoparticles (PS NPs) based on measurement fluorescent intensity of nanoparticles which is intracellular localized by flow cytometry method. It is also focus on determination of changes in cell cycle by PS NPs

2. Experimental
Cell analyzer SP6800 was used to anaysis cellular uptake ratio of green fluorescent labelled PS NPs. It was also used to assess changes in cell cycle under affecting of PS NPs

3. Results and Discussion
We were determined EGF enhanced cellular uptake of PS NPs by A431 cells

We were also determined PS NPs interfered to cell cycle of A431 cells. (A) control, (B) cells treated with PS NPs

4. Others
I have received helpful supports from Dr. Hattori and Ms. Morita in training to operate cell analyzer machiney.

5. Publication/Presentation
(1) Le Thi Minh Phuc, Akiyoshi Taniguchi. Epidermal growth factor enhances cellular uptake of polystyrene nanoparticles by clathrin-mediated endocytosis. Int. J. Mol. Sci. 2017, 18, 1301.

6. Patent
N/A