課題番号 ：S-19-NM-0001
利用形態 ：技術補助
利用課題名（日本語） ：ナノ材料の合成と生物医学的応用
Program Title (English) ：Synthesis and biomedical application of nanomaterials
利用者名（日本語） ：王秀鵬1) ，etc
Username (English) ：Xiupeng Wang1) ，etc.
所属名（日本語） ：1) 産業技術総合研究所 (AIST)
Affiliation (English) ：1) National Institute of Advanced Industrial Science and Technology (AIST)

１．概要（Summary ）
We used NIMS Molecule & Material Synthesis Platform to study the interaction between different nanomaterials and cells. The particle size and surface charge, drug/protein adsorption/release and cellular uptake of the different nanomaterials were studied.

２．実験（Experimental）
Equipments used in this year include レーザーゼータ電位計 ELSZ-1000, 動的光散乱（ダイナミック光散乱光度計）DLS-8000HAL, 共焦点レーザー顕微鏡 TCS SP5, etc. The experiment operation was guided by the specialist in NIMS Molecule & Material Synthesis Platform. Operation of the equipment was in accordance with the guidebook of the equipment.

３．結果と考察（Results and Discussion）
We synthesized hydroxyapatite nanorods with length of 100 nm, 200 nm, 500 nm, 1 μm and 10 μm in National Institute of Advanced Industrial Science and Technology (AIST). These hydroxyapatite nanorods were used as immunoadjuvant in AIST. Part of evaluation of these hydroxyapatite nanorods was supported by NIMS Molecule & Material Synthesis Platform.
For example, using共焦点レーザー顕微鏡 TCS SP5, we studied effect of size of hydroxyapatite nanorods on cancer antigen cellular uptake by dendritic cells. Free model cancer antigen was only slightly uptaken by dendritic cells. Hydroxyapatite nanorods markedly increased antigen cellular uptake by DCs. Among hydroxyapatite nanorods, the hydroxyapatite nanorods wiht length of 100-500 nm showed promoted antigen cellular uptake compared to thouse with length of 1-10 μm.

４．その他・特記事項（Others）
We thank Drs. Li Xianglan, Li Jie and Hiromi Morita about usage of the instrument.

５．論文・学会発表（Publication/Presentation）
1. Xiupeng Wang, Shu Ihara, Xia Li, Atsuo Ito, Yu Sogo, Yohei Watanabe, Atsushi Yamazaki, Noriko M. Tsuji, Tadao Ohno. Rod-Scale Design Strategies for Immune-Targeted Delivery System toward Cancer Immunotherapy. ACS Nano. 2019; 13: 7705-7715 .
2. Xia Li, Xiupeng Wang, Guowen Qian, Atsuo Ito. Synergistical Chemotherapy and Cancer Immunotherapy Using Dual Drug-delivering and Immunopotentiating Mesoporous Silica. Applied Materials Today. 2019; 16: 102-111.

６．関連特許（Patent）
なし