課題番号 ：S-19-NM-0011
利用形態 ：技術補助
利用課題名（日本語） ：
Program Title (English) ：Effect of serotonin receptors (5-HTR) on neural morphology
利用者名（日本語） ：アグラワル　ロケシュ
Username (English) ：Lokesh Agrawal (supervisior Prof. Takashi Shiga)
所属名（日本語） ：筑波大学大学院人間総合科学研究科
Affiliation (English) ：University of Tsukuba
検索キーワード（Keywords） ： Serotonin, Brain development, Scanning electron microscope, Hippocmpus

１．概要（Summary ）
Serotonin (5-HT) is known to play important role in the neural development, but specific role of 5-HT receptor is still poorly understood. Hence, we extensively investigated the role of 5-HT receptor in the development of hippocampal neurons in mice.

２．実験（Experimental）
We observed the effect of 5-HT4 receptor agonist on the neurite formation in hippocampal culture using immunohistochemistry and Scanning electron miroscope (SEM).

３．結果と考察（Results and Discussion）
We found that treatment with 5-HT4 receptor agonist promoted the neurite formation and branching in the embryonic mouse hippocampal neurons (Figs.1,2). These results could be useful for the treatment of neural developmental disorder like autism spectrum disorder and schizophrenia where 5-HT related-abnormal growth of neurons are observed.

Fig.1: SEM image of hippocampal Neurons.

Fig.2: 5-HT4R expression in mouse hippocampus in-vivo. Staining with anti-5-HT4R antibody (Green).

４．その他・特記事項（Others）
We would like to acknowledge Hattori San and Minowa san for their support. Additionally, we acknowledge our collaborators Dr. Sunil Kumar Vimal (Souhwest University, China). We also acknowledge Toshimi Otsuka Scholarship Foundation and Grant-in-Aid for Scientific Research (26640024, 17K08487) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan for the support during the study.

５．論文・学会発表（Publication/Presentation）
Publication
Agrawal L, Vimal SK, Shiga T. Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice. Neuropharmacology, 158, 107712, 2019 (ISSN: 0028-3908, doi.org/10.1016/j.neuropharm.2019.107712).

Presentations
1. Agrawal L, Shiga T. Promotion of axon and dendrite formation by serotonin 4 receptor through collapsing response mediator protein-2. Society for Neuroscience (SFN) meeting 2019, Chicago, US.
2. Agrawal L, Shiga T. Role of serotonin 4 receptor in neurite formation of hippocampal neurons. 9th ASJA annual symposium, AIST, Japan, 2019.

６．関連特許（Patents）
Not applicable.