Subject Number :S-18-MS-1067
Support Type : Technical support (except the training)

Proposal Title (English) : Magnetic property analysis of AgMNP-GRP
Username (English) : Jaewook Lee
Affiliation (English) : Department of Applied Biological Chemistry, Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.

1. Summary
The analysis revealed that the nanocomposite was synthesized while maintaining the properties of the magnetic nanoparticles. We will continue research aimed at the purification of target proteins from contaminating proteins using novel nanocomplexes.

2. Experimental
Nanoparticles decorated carbon nanotubes or graphenes have been spotlighted due to their synergic properties such as enhanced electrical property, magneto optical (MO) effect, plasmon resonance energy transfer (PRET) and so on. Indeed, silver (Ag)/magnetic nanoparticles (MNP) modified carbon nanomaterials possess synergic properties such as magnetic property and enhanced electrical conductivity.[1-3] In this study, I prepared multi-functional Ag/MNP-graphene for protein purification as magnetic carrier. (Fig. 1)

Fig 1. Schematic illustration of Ag/MNP-GRP based magnetic separation method

For this study, magnetic property of Ag/MNP-Graphene will be measured by using superconducting quantum interference device (SQUID, MPMS-XL7) in Institute for Molecular Science (IMS). In this case, the measurement condition that the magnetic-hysteresis relationship will be characterized from – 30 kOe to 30 kOe at room temperature. And from the MH hysteresis curve, the magnetic property of Ag/MNP-GRP will be investigated.

3. Results and Discussion
A nonlinear magnetic−hysteresis relationship and a reversible hysteresis loop were observed for the Ag/MNP-Graphen. The coercive force of the Ag/MNP-Graphene was measured to be approximately − 10 Oe and 10 Oe. On the other hand, remanence effects of approximately 0.06 and − 0.06 emu/g were indicated for the Ag/MNP-Graphene. According to this hysteresis loop, the GRP structure exhibited magnetic properties after hybridization with the MNPs. Additionally, this indicates that the Ag/MNP-Graphene were aligned under an external magnetic field.

Fig.2 The SQUID result of Ag/MNP-Graphene

4. Others
References
1. Jaewook Lee, and Jaebeom Lee, Magneto-Optically Active Magnetoplasmonic Graphene, Chemical Communications, 2017, 53, 5814. (back cover issue)
2. Jaewook Lee, et al., Binary Nanoparticle-graphene Hybrid Structure-based Highly Sensitive Biosensing Platform for Norovirus-like Particle Detection, ACS Applied Materials and Interfaces, 2017, 9, 27298.
3. Jaewook Lee*, et al, Magnetically aligned iron oxide/gold nanoparticles decorated carbon nanotube hybrid structure as humidity sensor, ACS Applied Materials & Interface, 2015, 7, 15506.