Subject Number : S-17-MS-1074
Support Type : Common use (including technical support necessary for the training)
Proposal Title (English) : Magnetic plasmonic graphene for fluoroassay
Username (English) :Jaewook Lee, Enoch Y. Park
Affiliation (English) :Research Institute of Green Science and Technology (RIGST), Shizuoka University.

1. Summary

Binary nanoparticles (NPs) modified graphene have been spotlighted due to their multi-functionalities and unique property. And hybrid carbon nanomaterials were widely utilized for sensing platforms to monitor the target biomolecules. In this study, plasmonic/magnetic nanoparticles decorated graphene (PMGRP) was prepared to utilize for magnetic separation and plasmonic substrate in a magnetofluoro immunosensing (MFIS) platform. Au/iron oxide nanoparticles (NPs) on the surface of graphene induced the synergic properties including plasmonic and magnetic property.

2. Experimental
For this study, magnetic property of PMGRP was measured by using superconducting quantum interference device (SQUID, MPMS-XL7) in Institute for Molecular Science (IMS). In this case, the magnetic-hysteresis relationship was characterized from – 30 kOe to 30 kOe at room temperature.

3. Results and Discussion
The magnetic property of PMGRP was measured using a SQUID. As a result, the remanence effect of PMGRP was indicated at approximately -11 emu/g and 11 emu/g according to the magnetic hysteresis curve of PMGRP. In addition, coercive force was analyzed at approximately 0.06 Oe, and -0.06 Oe, indicating that graphene possesses magnetic force after IONP decoration. Especially, target virus was combined with PMGRP via antigen-antibody (Ag-Ab) reaction and this hybrid structure was magnetically separated from impurities matrix. Moreover, MFIS process was successfully demonstrated that the limit of detection was estimated about 2.86 fg/ml with a high selectivity.

4. Others
This research was supported by Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Overseas Researcher (16P16361).

5. Publication/Presentation
(1) Jaewook Lee, Kenshin Takemura, Enoch Y. Park, Plasmonic/magnetic graphene-based magnetofluoro-immunosensing platform for virus detection, submitted.