Subject Number : S -16-MS-1091
Support Type : Common use (including technical support necessary for the training)
Proposal Title (English) : Magnetic property analysis for sensing platform
Username (English) :Jaewook Lee, Enoch Y. Park
Affiliation (English) :Research Institute of Green Science and Technology (RIGST), Shizuoka University.

1. Summary

Gold (Au)/magnetic nanoparticles (MNP) modified carbon nanomaterials possess synergic properties such as plasmonic/magnetic property, enhanced electrical conductivity, and magneto-optical (MO) effect. Thus, in our study, Au/MNP-graphene (AM-GRP) or Au/MNP-carbon nanotube (AM-CNT) based highly sensitive biosensing platform was developed for Noro Virus like particle (NoV-LP) detection or influenza virus DNA (InvDNA). Firstly, AM-GRP or AM-CNT were prepared by simple 2 steps and they was deposited on the Pt-electrode through external magnetic force in order to play a role as sensing channel. In this case, detection limitation of this sensing platform for NoV-LP was 0.01 pg/ml and its sensitivity for InvDNA was 1 pM. Especially their selectivity was also excellent.

2. Experimental
For this study, magnetic property of AM-GRP and AM-CNT were measured by using superconducting quantum interference device (SQUID, MPMS-XL7) in Institute for Molecular Science (IMS). In this case, the magnetic-hysteresis relationship was characterized from – 30 kOe to 30 kOe at room temperature.

3. Results and Discussion
As a result, a nonlinear and reversible hysteresis loop was obtained. According to the loop curve, remanence effect of AM-GRP was shown as 0.07 and -0.07 emu/g, approximately. Indeed, its coercive force was about -20 Oe and 20 Oe. On the other hands, remanence effect of AM-CNT was measured around 0.06 and -0.06emu/g. In addition, coercive force of AM-CNT was indicated around -10 Oe and 10 Oe. It meant that GRP could possesses a magnetic property after the MNP decoration process. Moreover, AM-CNT could be aligned following to the external magnetic fields.

4. Others
This research was supported by Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Overseas Researcher (16F16361).

5. Publication/Presentation
(1) J. Lee, K. Takemura, C. N. Kato, T. Suzuki, E. Y. Park*, Binary Nanoparticle-graphene Hybrid Structure-based Highly Sensitive Biosensing Platform for Norovirus-like Particle Detection, submitted
(2) J. Lee, M. Morita, K. Takemura, E. Y. Park*, Influenza DNA detection via gold/magnetic nanoparticles functionalized CNT based biosensing platform, submitted.
(3) J. Lee, M. Morita, K. Takemura, E. Y. Park*, Iron oxide/gold nanoparticles decorated graphene for biosensing application, presentation in 69th Annual Meeting of the Society for Biotechnology, Japan.(Sep. 11-14, 2017)
(4) J. Lee, and E. Y. Park* Iron oxide/gold nanoparticles decorated carbon nanomaterials for sensing application, presentation in The 8th Japen-China-Korea joint Conference on MEMS/NEMS, Korea (July 14. 2017)