Subject Number : S-19-NM-0073
Support Type : 機器利用
Proposal Title (English) : Penetration of fluorescent compound by beauty device in ex vivo skin model
Username (English) : LIU Shu
Affiliation (English) : NIHON LOREAL K.K

1. Summary

The permeation of fluorescent compounds into the excised human skin was investigated with and without the combination of the beauty device.
Fluorescent compounds applied with the device were observed to permeate deeper into skin than the fluorescent compounds topical solution. Moreover, the permeation of the fluorescent compounds applied with the device showed obvious dependency on the molecule weight of the fluorescent compound. While the permeation of the fluorescent compounds with the topical solution is only on the surface of the human skin, without any dependency on the molecule weight of the compound.

2. Experimental

The excised human skin was prepared before the day. After 30 minutes in room temperature, the human skin was applied with the five simplex solutions, which contains the fluorescent compounds with different molecule weight as the control groups. In parallel, in the device groups, the human skin was first treated with the device and then applied with the five simplex solutions. After 15 minutes, the skin sample of all the groups was wiped with dry cotton swab and excised for frozen block preparation. After embedding in O.C.T compound, the skin samples were frozen in the liquid nitrogen for 10 minutes. 20 continuous histological cross-sections with the thickness of 10 µm were sliced from each frozen blocks with Cryostat at -15 ºC. All of the histological cross-section of the skin were observed with the confocal laser fluorescence microscope to investigate the penetration depth of the fluorescent compounds into skin.

3. Results and Discussion

All of the topical solution groups show that the fluorescent compounds are only on the surface of the skin and no permeation into epidermis and dermis layer. While with the device, the permeation of the fluorescent compounds is not only on the skin surface, but also permeated into deeper layer, i.e. epidermis. And the permeation of the fluorescent compounds shows the molecule weight dependency. Smaller compound permeated more and deeper into skin than the bigger ones.

4. Others
Dr. Li Xianglan provided enough support for us.

5. Publication/Presentation
N/A

6. Patent
N/A